pVL1392,pVL1393TransferVectorSet

pVL1392andpVL1393aretransfervectorsdesignedforhigh-levelexpressionofaclonedgenedrivenbythestrongAutographacalifornicamultiplenucleopolyhedrovirus(AcMNPV)polyhedrinpromoter(pPolh).

Description:

pVL1392andpVL1393arepUC8basedplasmidscontainingtheAcMNPVpolyhedrinlocuslackingthemajorityofthepolyhedrinopenreADIngframe.Amultiplecloningsite(MCS)sequencehasbeeninserted37nucleotidesdownstreamfromtheoriginalpolyhedrinATGstartcodon,whichhasbeenmutatedtoATT.

pVL1392andpVL1393shareidenticalsequences,withtheexceptionthattheyeachhavethesameMCSinopposingorientations.AstartandstopcodonmustbeprovidedwiththegenethatistobeclonedintotheMCS.Formaximalrecombinantproteinexpression,thedistancebetweenthecloningsiteandtheATGstartoftheinsertedgeneshouldbekepttoaminimum,andshouldnotexceed100nucleotides.

Specification:

MediaType	ESF921andESFAF
PlatformCompatibility	BestBac1.0andBestBac2.0
Shippingcondition	Ambient
Storagecondition	2-8°C,protectfromlight

RecommendedStorageCondition

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