pVL1392TransferVector

pVL1392andpVL1393aretransfervectorsdesignedforhigh-levelexpressionofaclonedgenedrivenbythestrongAutographacalifornicamultiplenucleopolyhedrovirus(AcMNPV)polyhedrinpromoter(pPolh).

Description:

pVL1392andpVL1393arepUC8basedplasmidscontainingtheAcMNPVpolyhedrinlocuslackingthemajorityofthepolyhedrinopenreADIngframe.Amultiplecloningsite(MCS)sequencehasbeeninserted37nucleotidesdownstreamfromtheoriginalpolyhedrinATGstartcodon,whichhasbeenmutatedtoATT.

pVL1392andpVL1393shareidenticalsequences,withtheexceptionthattheyeachhavethesameMCSinopposingorientations.AstartandstopcodonmustbeprovidedwiththegenethatistobeclonedintotheMCS.Formaximalrecombinantproteinexpression,thedistancebetweenthecloningsiteandtheATGstartoftheinsertedgeneshouldbekepttoaminimum,andshouldnotexceed100nucleotides.

Specification:

MediaType	ESF921andESFAF
PlatformCompatibility	BestBac1.0andBestBac2.0
Shippingcondition	Ambient
Storagecondition	2-8°C,protectfromlight

RecommendedStorageCondition

2–8ºC Expression Systems总部位于加利福尼亚州戴维斯，致力于为杆状病毒，细胞培养和生物工业市场提供创新的培养基配方，互补产品和合同服务，并为其提供服务。我们专注于杆状病毒/宿主细胞系统，重点是昆虫细胞和哺乳动物细胞系的昆虫细胞培养和哺乳动物细胞培养。杆状病毒表达载体系统我们对BEVS应用程序的独特关注可帮助全球学术，生物技术和制药领域的研究人员和临床医生以最高的一致性工作。我们的产品和服务被世界各地的精英机构，公司和实验室使用。从基因合成到蛋白质纯化，在此过程的任何步骤中，Expression Systems都能帮助您获得最佳结果。