Product Specification:
| Alternative Name: | Glutamate receptor 1, AMPA-selective glutamate receptor 1, Glutamate receptor ionontropic AMPA 1 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 271-285 of rat GluR1 (GRIA1). |
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| UniProt ID: | P19490 |
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| Species reactivity: | Mouse, Rat
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| Applications: | IHC, WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (1:100) Western Blot (1:200) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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| Blocking Peptide: | Included: GluR1 (extracellular) Control Peptide (100µg). Supplied lyophilized.
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| Purity Detail: | Affinity purified |
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| Formulation: | Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide. |
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| Reconstitution: | To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. Further dilutions should be made using a carrier protein such as 1% BSA. To lyophilized control peptide, add 100µl of distilled water. |
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| Use/Stability: | Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | GluR receptors are permeable to cations Na and K and Ca2+. Gating of Glu receptors by glutamate is extremely fast and therefore the Glu receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. |
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| Technical Info/Product Notes: | PREADSORPTION CONTROL: 1µg control peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments.
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西安凯新生物科技有限公司还是ENZO lifesciences公司中国区代理商,eBioscience公司中国区代理商,Biolegend公司中国区代理商,Ludger司中国区代理商,Peprotech公司中国区代理商,anaspec公司中国区代理商,CST公司中国区代理商,Prospec 中国区代理商,Innova Biosciences 中国区代理商,abcam代理商,BD Biosciences公司中国区代理商,Abnova公司中国区代理商,Cayman Chemical公司的中国区代理。
Enzo旗下共有Enzo,Biomol,Alexis Biochemicals,AssayDesigns,Stressgen等品牌,为生命科学研究者提供基因组分析,细胞生物学,翻译后修饰,信号转导,肿瘤和免疫学,药物筛选等相关领域的优质产品。Enzolife sciences 的标记技术和检测技术在科学研究和临床诊断这两个领域中处于领先地位。标记探针和染料的强强组合是基因表达分析,核酸检测,蛋白质生物化学研究与检测,分子生物学,细胞分析等研究领域中目标识别/验证和高容量分析实验的强有力工具。
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