Product Description

Normal human serum depleted of complement C2 protein by immunoaffinity chromatography. The product is tested for the absence of C2 by functional assays for classical pathway activity and for C2 protein by double immunodiffusion. C2-Dpl is certified to possess a functional alternative pathway for complement activation (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)).A functional classical pathway can be reconstituted by addition of purified C2 protein (25 µg/mL) indicating that all other complement components necessary for classical pathway activation are present.The absence of C2 also prevents complement activation by the lectin pathway but the alternative pathway will still activate on appropriate surfaces.

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。