Product Description

Normal human serum is tested for complement activity and certified to possess fully functional classical and alternative pathways of activation. Our NHS is also referred to in our product descriptions as the NHS complement standard.A functional lectin pathway is assumed to be present, but standard assays for all of its initiating components (ficolins, MASPs, and MBL) have not been established.Therefore, we have not tested specifically for lectin pathway activity.

NHS was prepared from a large number of donors.Each unit of blood was collected without anticoagulants and each was individually tested for transfusion-transmissible diseases before pooling (see Precautions/Toxicity/Hazards below).After coagulation, the liquid portion was separated by centrifugation.The different units were pooled, filtered through a 0.22 µm filter, aliqoted and frozen at -80^oC.

All testing for complement activity was performed on the subsequently thawed samples to guarantee that the functional activity reported is what customers will receive when the samples are thawed.Complement activity is stable for several years if NHS is stored at -70^oC or below continuously.A slight degradation of activity may be observed following additional freeze thaw cycles.

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。