Product Description

Normal human serum depleted of complement C9 protein by immunoaffinity chromatography. The product is tested for the absence of C9 by functional assays for classical pathway activity and alternative pathway activity and for C9 protein by double immunodiffusion. C9-Dpl is certified to possess functional classical and alternative pathways for complement activation up to the step of C9 binding to C5b678 (Law, S.K.A. and Reid, K.B.M. (1995); Morgan, B.P. ed. (2000); Dodds, A.W. and Sim, R.B. editors (1997)).Functional complement systems can be reconstituted by addition of purified C9 protein (60 µg/mL) indicating that all other complement components necessary for complement activation are present.Note that the C5b678 complex has considerable hemolytic potential without C9 and therefore C9-Dpl is capable of lysing EA and most other cells directly without added C9 if activation is strong enough and if the concentration of depleted serum used is high enough.

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。