Product Description

Natural human C3a is prepared by cleavage of human C3 protein by a human C3 convertase. C3a is a member of the anaphylatoxin family of three proteins (C3a, C4a and C5a) produced by the activation of complement.It is an unglycosylated polypeptide containing 77 amino acids with a molecular mass of 9,089 daltons.Many of the biological functions of C3a are similar to those of C5a, but C3a is approximately 10- to 20-fold less active per microgram than C5a. C3a mediates many inflammatory responses including smooth muscle contraction, vasodilation, increased vascular permeability, and release of histamine from mast cells and basophils (Law, S.K.A. and Reid, K.B.M. (1995)). In contrast to C5a, C3a does not exhibit significant neutrophil stimulating activities and does not induce chemotaxis, granule release or superoxide production.C3a acts through the C3a Receptor (C3aR) which is a G-protein coupled receptor found widely distributed on peripheral tissues, lymphoid cells (neutrohphils, monocyes, and eosinophils) and in the central nervous system (astrocytes, neurons and glial cells) (Law, S.K.A. and Reid, K.B.M. (1995)).These activities of C3a are inactivated by removal of the C-terminal arginine and this occurs rapidly in plasma due to the action of carboxypeptidase N (Meuller-Ortiz, S.L., et al. (2009)).

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。