Product Description

C3/FB-Depleted Serum is normal humanserum in which C3 and factor B(fB) have been removed by immunoaffinity chromatography.The product istested for the absence of C3 and factor B activity by testing classical and alternative pathway function and for the absence of C3 andfactorB proteins by double immunodiffusion. The C3/FB-depleted serumis certified to exhibitless than 5% classical pathway and alternativepathway activities. A functionalclassical pathway can be reconstitutedby addition of purified C3 protein (1.3mg/mL) whereas a functionalalternative pathway can be reconstituted byaddition of purified C3protein (1.3 mg/mL) and factor B protein (0.2 mg/mL)indicating thatall other complement components necessary for classical andalternativepathway activation are present. After reconstitution with C3 (1.3mgC3/mL) the C3/FB-Depleted Serum is certified to possess a functional classical pathway for complement activation whereas after reconstitutionwithC3 (1.3 mg C3/mL) and factor B (0.2 mg/mL), the product iscertified to possessa functional alternative pathway for complementactivation (Morgan, B.P.(2000); Dodds, A.W. and Sim, R.B. (1997)).Although a functional lectin pathwayshould also be reconstituted bythe addition of similar concentrations of C3,the function of thispathway is not tested.

Note that there is a weak C3by-passmechanism whereby the convertase C4b,C2a is able to activate C5(Rawal, N.& Pangburn, M.K. 2003) and form C5b-9 complexes capableof causing lowgrade lysis. This system of C5 activation is functionalin both the classicaland the lectin pathways and is approximately1000-fold less active than in thepresence of C3.

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。