Product Description

C1q/Factor D-depleted serum is normal human serum in which complement proteins C1q and factor D have been depleted by ion exchange and immunoaffinity chromatography. The product is tested for C1q and factor D proteins by double immunodiffusion and the absence of C1q and factor D is measured by testing classical and alternative pathway function. The product is certified to exhibit less than 5% classical and alternative pathway activity. After reconstitution with purified C1q protein (70 µg/mL), C1q/Factor D-depleted serum is certified to possess a functional classical pathway for complement activation (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)).Similarly, a functional alternative pathway can be reconstituted by addition of purified factor D (1.4 µg/mL) indicating that all other complement components necessary for alternative pathway activation are present. Lectin pathway activity is not tested, but it would be expected to be active.

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。