Product Description

Normal human serum was depleted of factor H by immunoaffinity chromatography.The product is tested for the absence of factor H by double immunodiffusion. Factor H is a regulator of complement activation.As a result, factor H-Dpl is still capable of activating the alternative pathway and does so spontaneously and very rapidly in the absence of factor H.In fact, activation occurs in less than 2 min in the fluid phase without an activating particle resulting in the consumption of both C3 and factor B.For this reason this depleted serum is stored with 0.1 mM EDTA to inhibit spontaneous activation.Factor H-Dpl is certified to possess a functional alternative pathway for complement activation only if a controlling factor, such as factor H, is added prior to the addition of metal ions (specifically Mg⁺⁺).Full reconstitution requires addition of 500 µg factor H/mL serum.It is also tested for and certified to contain functional classical pathway indicating that all other complement components necessary for classical and alternative pathway activation are present except for factor H (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)).

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。