Product Description

C1s depleted serum (C1s-Dpl) is normal human serum depleted of C1s byimmunoaffinity chromatography. The product is tested for the absence ofC1s by functional assays for classical pathway activity and for C1sprotein by double immunodiffusion. C1s-Dpl is certified to possess afunctional alternative pathway for complement activation (Morgan, B.P.(2000); Dodds, A.W. and Sim, R.B. (1997)).

In serumC1s is present in its proenzyme form (Valet, G and Cooper N.R. (1974);Ziccardi, R.J. and Cooper N.R. (1976)). C1s enzyme is the activated formof C1s proenzyme. C1s is a subunit of the C1 complex which is the firstcomponent in the classical pathway of complement (See productdescription for C1 Cat# A098). C1s proenzyme is an inactive zymogenuntil C1 is activated. C1r is activated when C1 binds to and isactivated by antibodies bound to antigens (immune complexes) yieldingC1r enzyme, the first protease that initiates the cascade. C1r enzyme inthe C1 complex activates C1s proenzyme generating C1s enzyme. Afunctional classical pathway can be reconstituted by addition ofpurified C1s proenzyme (31 µg/mL) to the C1s-Dpl indicating that allother complement components necessary for classical pathway activationare present and active.

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。