Product Description

Factor Ba is the fragment of complement factor B that results from activation of the alternative pathway.CompTech prepares the factor Ba fragment from factor B which was purified from normal human serum.Complement factor B is a glycosylated protein composed of a single 93,000 Da polypeptide chain.Factor B is an essential component of the alternative pathway of complement activation and is found in plasma at approximately 200 µg/mL.In the presence of Mg⁺⁺ factor B binds to C3b and the C3b,B complex can be activated by factor D, a serine protease that circulates as an active trypsin-like serine protease.Cleavage of factor B by factor D causes the release of the Ba fragment (33,000 Da) and leaves the 60,000 Bb fragment bound to C3b.This Ba fragment comes from the N-terminal of factor B and it contains three CCP domains which interact with C3b (Morley, B.J. and Walport, M.J. (2000)). The isolated fragment Ba has been reported to have a weak affinity for C3b and to inhibit the interaction of factor B with C3b thus inhibiting the activation of the alternative pathway (Pryzdial, E.L. and Isenman, D.E., (1987)).

The fragments of factor B (Ba and Bb) have been proposed to elicit several biological responses.See the section titled Function below and in the product description for the Bb fragment.

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。