Product Description

iC3b (inactivated C3b) is derived from C3b.Conversion of C3b to iC3b destroys almost all of the functional binding sites present on C3b.C3b itself is produced by all three pathways of complement (Law, S.K.A. and Reid, K.B.M. (1995)) when native C3 is cleaved releasing C3a.iC3b is prepared by cleavage of C3b by factor I in the presence of factor H.Cleavage by factors H and I occurs rapidly when the C3b is free in solution and is slower when it is attached to a surface.Other cofactors for factor I also permit cleavage if C3b to iC3b and these include the two membrane proteins CR1 (CD35) and MCP (CD46).Factor I can cleave C3b in two places in the alpha chain and if both sites are cleaved a small fragment (C3f, 2,000 Da) is released.Thus, iC3b may be a mixture of 176,000 and 174,000 molecular weight proteins.If the C3b precursor was attached to a surface, the iC3b remains on that surface.The iC3b sold by CompTech is made from fluid phase C3b and is not capable of attaching to a surface.Surface-bound C3b and iC3b are linked to the target through a covalent bond which may be either an ester bond or an amide bond.Ester bonds are unstable resulting in the gradual release from the particle.Most of the C3b generated during complement activation never attaches to a surface because its thioester reacts with water forming fluid phase C3b.Surface-bound iC3b and its breakdown product C3d are recognized by numerous receptors on lymphoid and phagocytic cells which use these ligands to stimulate phagocytosis and antigen presentation to cells of the adaptive immune system.Receptors for iC3b are CR2 (CD21) found on B-cells and CR3 (CD11b/CD18) found on phagocytes (Dodds, A.W. and Sim, R.B. editors (1997); Morley, B.J. and Walport, M.J. (2000)).One of the results of iC3b-receptor interaction is an expansion of target-specific B-cell and T-cell populations.

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。