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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 105922 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
| AAV2 | 105922-AAV2 | 100 µL at titer ≥ 7×10¹² vg/mLand Plasmid.More Information | Add to Cart | |||
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Modifications to backbone11bp deletion (aaagcccgggc) in left ITR removes 1 SmaI site
- Vector typeAAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature30°C
- Growth Strain(s)NEB Stable
- Copy numberUnknown
Gene/Insert1
- Gene/Insert namemKate2
- Mutationcodon optimized
- PromoterCBh
Cloning Informationfor Gene/Insert 1
- Cloning methodRestriction Enzyme
- 5′ cloning siteSalI(not destroyed)
- 3′ cloning siteEcoRI(not destroyed)
- 5′ sequencing primerpCBhProF2 (5-agggtttaagggatggttgg)
- 3′ sequencing primerpCDH-rev (5-GCATTCCTTTGGCGAGAG) (Common Sequencing Primers)
Gene/Insert2
- Gene/Insert nameIRES-Multiple cloning site
- Alt nameMCS is FseI-PacI-SpeI-BamHI-XhoI-StuI-MluI-NheI-BmtI-AsiSI-AscI
- Promotersame CBh promoter as mKate2
Cloning Informationfor Gene/Insert 2
- Cloning methodRestriction Enzyme
- 5′ cloning siteN/A(unknown if destroyed)
- 3′ cloning siteN/A(unknown if destroyed)
- 5′ sequencing primerIRES-F (5-TGGCTCTCCTCAAGCGTATT)
- 3′ sequencing primerWPRE-R (5-CATAGCGTAAAAGGAGCAACA) (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- pAAV-CBh-mKate2-IRES-MCS Cgap WT final.gb
- Terms and Licenses
- UBMTA
- Evrogen Limited Use Label License for FPs
- Industry Terms
- Not Available to Industry
Depositor Comments
Entire transgene cassette can be removed with NotI/XbaI digest; CBh promoter can be removed by XbaI/AgeI digest; mKate2 can be removed with SaI/EcoRI digest; IRES can be removed by EcoRI/FseI digest
Information for AAV2 (Catalog # 105922-AAV2)(Back to top)
Purpose
Ready-to-use AAV2 particles produced from pAAV-CBh-mKate2-IRES-MCS (C-gap-WT) (#105922). In addition to the viral particles, you will also receive purified pAAV-CBh-mKate2-IRES-MCS (C-gap-WT) plasmid DNA.
Control mKate2 expression AAV with one mutated and one wild-type ITR. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV2
- PurificationIodixanol gradient ultracentrifugation
- Reporter GenemKate2
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
