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Abnova/mutaFISH™ DNA Accessory Kit 1 for Cells/1kit/KA4916

价格
面议
货号:KA4916
浏览量:127
品牌:Abnova
服务
全国联保
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正规发票
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商品描述
  • Specification
  • Product Description:
  • mutaFISH™ DNA Accessory Kit 1 for Cells provides reagents and enzymes for in situ restriction digestion, exonucleolysis, mutaFISH™ hybridization, ligation and amplification in cell prior to mutaFISH™.
  • Supplied Product:
  • Box 1 content:1. H2O2. PBS3. Wash Buffer 14. Digestion Enzyme Buffer5. Wash Buffer 26. Formamide7. Detection BufferBox 2 content:1. Digestion Enzyme2. BSA3. Restriction Enzyme 14. 10X Restriction Enzyme Buffer5. Hybrid Enzyme6. 10X Hybrid Enzyme Buffer7. 1M KCL8. DNA Polymerase9. 10X DNA Pol Buffer10. dNTP Mix11. 50% Glycerol12. DAPI (100uM)
  • Storage Instruction:
  • Store Box 1 at 4°C, store Box 2 at -20°C.
  • Protocol:
  • PDF DownloadProtocol Download
  • Technology:
  • mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
  • Comparison:
  • FISH Probes vs mutaFISH™ Probes
  • Regulatory Status:
  • For research use only (RUO)
  • Video:
  • Datasheet:
  • PDF DownloadDownload
  • Applications
  • mutation specific, Fluorescence In Situ Hybridization (Cells)
  • mutation specific, Fluorescence <em>In Situ</em> Hybridization (Cells)
  • The figure represents an example workflow of detecting a point mutation on double strand DNA by using mutaFISH™ probes with mutaFISH™ DNA Accessory Kit.
  • Application Image
  • mutation specific, Fluorescence In Situ Hybridization (Cells)
  • mutation specific, Fluorescence <em>In Situ</em> Hybridization (Cells)
  • enlarge
Abnova的Abnova Diagnostics F蛋白表达系统基于小麦胚芽的真核翻译设备。小麦胚在浓缩干燥状态下储存所有翻译成分,一旦发芽就准备好进行蛋白质合成。常规的小麦胚芽提取物包含RNA N-糖苷酶三丁酸酯和其他翻译抑制剂,例如硫蛋白,核糖核酸酶,脱氧核糖核酸酶和蛋白酶。这些抑制剂来自胚乳。大量洗涤小麦胚芽以消除胚乳污染物已导致提取物具有高度的稳定性和活性。通过将具有5'cap和多(RSA)尾巴的mRNA与该提取物与专有脂质体结合使用,进行体外翻译反应产生足够量的膜蛋白,该膜蛋白被脂质体捕获,导致正确的构象和生物学功能必不可少的折叠。该系统相对于普遍使用的蛋白质表达平台(例如大肠杆菌,昆虫细胞和哺乳动物细胞)具有明显优势,这些平台都是体内系统,并且在细胞膜的约束下导致产量和稳定性低。此外,体外小麦胚芽系统适于自动化以用于高通量小分子以及生物学筛选和表征。