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Abnova/ACTB monoclonal antibody, clone BF-1/1kit/MAB19613

价格
面议
货号:MAB19613
浏览量:127
品牌:Abnova
服务
全国联保
正品保证
正规发票
签订合同
商品描述
  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human ACTB.
  • Immunogen:
  • A synthetic peptide corresponding to human ACTB.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunocytochemistry (1:50-1:200)Immunofluorescence (1:50-1:200)Immunohistochemistry (1:50-1:200)Western Blot (1:3000-1:10000)The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Datasheet:
  • PDF DownloadDownload
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1)HeLa cell lysate; (2)Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate with ACTB monoclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded (1) Human heart; (2) Mouse testis; (3) Human bladder cancer; (4) Mouse heart with ACTB monoclonal antibody.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent analysis of Hela cells with ACTB monoclonal antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 60
  • Protein Accession#:
  • P60709
  • Gene Name:
  • ACTB
  • Gene Alias:
  • PS1TP5BP1
  • Gene Description:
  • actin, beta
  • Omim ID:
  • 102630
  • Gene Ontology:
  • Hyperlink
  • Gene Summary:
  • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
  • Other Designations:
  • PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
  • Gene Pathway
  • Adherens junction
  • Arrhythmogenic right ventricular cardiomyopathy (ARVC)
  • Focal adhesion
  • Hypertrophic cardiomyopathy (HCM)
  • Leukocyte transendothelial migration
  • Pathogenic Escherichia coli infection - EHEC
  • Regulation of actin cytoskeleton
  • Tight junction
  • Vibrio cholerae infection
  • Related Disease
  • Breast cancer
  • Breast Neoplasms
Abnova的Abnova Diagnostics F蛋白表达系统基于小麦胚芽的真核翻译设备。小麦胚在浓缩干燥状态下储存所有翻译成分,一旦发芽就准备好进行蛋白质合成。常规的小麦胚芽提取物包含RNA N-糖苷酶三丁酸酯和其他翻译抑制剂,例如硫蛋白,核糖核酸酶,脱氧核糖核酸酶和蛋白酶。这些抑制剂来自胚乳。大量洗涤小麦胚芽以消除胚乳污染物已导致提取物具有高度的稳定性和活性。通过将具有5'cap和多(RSA)尾巴的mRNA与该提取物与专有脂质体结合使用,进行体外翻译反应产生足够量的膜蛋白,该膜蛋白被脂质体捕获,导致正确的构象和生物学功能必不可少的折叠。该系统相对于普遍使用的蛋白质表达平台(例如大肠杆菌,昆虫细胞和哺乳动物细胞)具有明显优势,这些平台都是体内系统,并且在细胞膜的约束下导致产量和稳定性低。此外,体外小麦胚芽系统适于自动化以用于高通量小分子以及生物学筛选和表征。