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Abnova/15-deoxy-Delta12,14-PGJ2 ELISA Kit/1kit/KA0303

价格
面议
货号:KA0303
浏览量:127
品牌:Abnova
服务
全国联保
正品保证
正规发票
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商品描述
  • Specification
  • Product Description:
  • 15-deoxy-Delta12,14-PGJ2 ELISA Kit is a competitive immunoassay for the quantitative determination of 15-deoxy-Delta12,14-Prostaglandin J2 (15-d-PGJ2) in biological fluids.
  • Calibration Range:
  • 195 to 200000 pg/mL
  • Limit of Detection:
  • 36.8 pg/mL
  • Quality Control Testing:
  • Standard curveQC Testing of KA0303The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
  • Storage Instruction:
  • Store all component at 4°C, except standard and conjugate at -20°C
  • Protocol:
  • PDF DownloadProtocol Download
  • MSDS:
  • PDF DownloadDownload
  • Suitable Sample:
  • Plasma, Saliva, Tissue culture media and Urine
  • Sample Volume:
  • 100 uL
  • Label:
  • AP-conjugate
  • Detection Method:
  • Colorimetric
  • Intra-Assay:
  • 6.23%
  • Inter-Assay:
  • 14.40%
  • Spiking Recovery:
  • Cell Culture Supernatant: 97.40%, Plasma: 105.00%, Saliva: 94.40%, Urine: 113.90%
  • Regulation Status:
  • For research use only (RUO)
  • Datasheet:
  • PDF DownloadDownload
  • Publication Reference
  • 1.
  • Chronic Methylmercury Exposure Induces Production of Prostaglandins: Evidence From A Population Study and A Rat Dosing Experiment.Kong HK, Gan CF, Xiong M, Kwok KW, Lui GC, Li P, Chan HM, Lo SC.Environ Sci Technol. 2019 Jul 2;53(13):7782-7791. doi: 10.1021/acs.est.9b00660. Epub 2019 Jun 21.
  • 2.
  • Antimycotics suppress the Malassezia extract-induced production of CXC chemokine ligand 10 in human keratinocytes.Hau CS, Kanda N, Makimura K, Watanabe SJ Dermatol. 2014 Feb;41(2):124-34. doi: 10.1111/1346-8138.12380.
  • 3.
  • Suppressive effects of antimycotics on thymic stromal lymphopoietin production in human keratinocytes.Hau CS, Kanda N, Watanabe SJ Dermatol Sci. 2013 May 2. pii: S0923-1811(13)00143-6. doi: 10.1016/j.jdermsci.2013.04.023.
  • Applications
  • Quantification
  • Application Image
  • Quantification
Abnova的Abnova Diagnostics F蛋白表达系统基于小麦胚芽的真核翻译设备。小麦胚在浓缩干燥状态下储存所有翻译成分,一旦发芽就准备好进行蛋白质合成。常规的小麦胚芽提取物包含RNA N-糖苷酶三丁酸酯和其他翻译抑制剂,例如硫蛋白,核糖核酸酶,脱氧核糖核酸酶和蛋白酶。这些抑制剂来自胚乳。大量洗涤小麦胚芽以消除胚乳污染物已导致提取物具有高度的稳定性和活性。通过将具有5'cap和多(RSA)尾巴的mRNA与该提取物与专有脂质体结合使用,进行体外翻译反应产生足够量的膜蛋白,该膜蛋白被脂质体捕获,导致正确的构象和生物学功能必不可少的折叠。该系统相对于普遍使用的蛋白质表达平台(例如大肠杆菌,昆虫细胞和哺乳动物细胞)具有明显优势,这些平台都是体内系统,并且在细胞膜的约束下导致产量和稳定性低。此外,体外小麦胚芽系统适于自动化以用于高通量小分子以及生物学筛选和表征。