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蚂蚁淘在线 / 品牌中心 / Abnova / Abnova/A-431 (human epidermoid carcinoma) whole cell lysate (non-denatured)/1kit/L015V2
商品描述
- Specification
- Product Description:
- Whole cell lysate (non-denatured)
- Tissue:
- Epidermis
- Host:
- Human
- Preparation Method:
- Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
- Lysis Buffer:
- Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
- Quality Control Testing:
- 12.5% SDS-PAGE Stained with Coomassie Blue.SDS-PAGE Gel
- Recommend Usage:
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Storage Buffer:
- In modified RIPA Lysis Buffer.
- Storage Instruction:
- Store at -80°C. Aliquot to avoid repeated freezing and thawing.
- Datasheet:
Download
- Publication Reference
- 1.
- Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays.Yang Y, Zeng Y.Lab Chip. 2018 Dec 4;18(24):3830-3839. doi: 10.1039/c8lc00927a.
- Applications
- Western Blot
- Immunoprecipitation
- Protocol Download
- Application Image
- Western Blot
- Immunoprecipitation
