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蚂蚁淘在线 / 品牌中心 / Abnova / Abnova/SARS-CoV-2 S ECD recombinant human antibody, clone BHB05/1kit/RAB01049
商品描述
- Specification
- Product Description:
- Recombinant human antibody raised against SARS-CoV-2 S ECD.
- Immunogen:
- Natural SARS-CoV-2.
- Host:
- Human
- Reactivity:
- SARS-CoV-2
- Specificity:
- This antibody detects endogenous levels of S-ECD protein.
- Form:
- Liquid
- Purification:
- Protein A purification
- Purity:
- > 95% (by SDS-PAGE).
- Isotype:
- IgG
- Recommend Usage:
- ELISA (1:80000)NeutralizationWestern Blot (1:3000-1:4000)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS, pH 7.2.
- Storage Instruction:
- Store at 4°C for short term. For long term storage, store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Antibody Species:
- Human
- Source / Expression System:
- 293F
- Matched Pair:
- RAB01045 with RAB01049; RAB01046 with RAB01049
- Datasheet:
Download
- Applications
- Neutralization
- Western Blot (Recombinant protein)
- Western Blot analysis of Lane 1: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) 1 ug and Lane 2: S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 1 ug. Primary antibody: SARS-CoV-2 S ECD recombinant human antibody, clone BHB05 (Cat # RAB01049) (1:4000). Secondary antibody: Goat anti-human IgG+HRP (1:5000). Exposure time: 120 s.
- Enzyme-linked Immunoabsorbent Assay
- An ELISA was performed using 1. Coating original concentration: 2 ug/mL, 100 uL/hole sample is S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) and S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 2. Primary antibody: SARS-CoV-2 S ECD recombinant human antibody, clone BHB05 (Cat # RAB01049) 3. Secondary antibody: Goat anti-human IgG+HRP (1:10000) 4. Develop: 15 min, 100 uL/hole 5. Stop: stop buffer 50 uL/hole 6. ELISA result are shown in the figure.
- Application Image
- Neutralization
- Western Blot (Recombinant protein)
- enlarge
- Enzyme-linked Immunoabsorbent Assay
- enlarge
