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商品描述
Human Anti-Cetuximab Antibody, clone AbD19376_hIgG1, is a non-inhibitory anti-idiotypic antibody that binds to cetuximab. This antibody recognizes an epitope outside the cetuximab binding site and so does not inhibit the binding of the drug to its target, epidermal growth factor receptor (EGFR). This epitope is shared with other chimeric antibodies that are highly homologous to cetuximab including infliximab but excluding rituximab. The antibody is suitable for the development of bioanalytical assays for cetuximab and biosimilars.Clone AbD19376_hIgG1 is a fully human recombinant monoclonal with IgG1 isotype and is suitable as a surrogate positive control or calibrator in an anti-drug antibody (ADA) assay. In addition, it is suitable as a detection antibody in pharmacokinetic (PK) bridging ELISA, with Anti-Cetuximab Antibody HCA220 as the capture antibody. This combination enables measurement of free and partially bound cetuximab, which is equivalent to total drug when trough serum levels are above 4 μg/ml.Cetuximab (brand name Erbitux) is a chimeric monoclonal antibody drug (IgG1/kappa) approved for treatment of KRAS wild-type metastatic colorectal cancers and squamous cell carcinoma of the head and neck. This therapeutic antibody is directed against EGFR and inhibits tumor cell proliferation by blocking the interaction of epidermal growth factor (EGF) with its receptor.View a summary of all anti cetuximab antibodies.
Product Details
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Chimeric monoclonal antibody
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=39 nM by real time, label-free molecular interaction analysis on immobilized cetuximab.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -70oC.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
- Storage
- Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Cetuximab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as a detection antibody in a cetuximab bridging ELISA for PK assay development together with HCA220 as the capture reagent.Protocol: PK bridging ELISA to measure total drug
- ELISA
- This product may be used in a direct ELISA or when conjugated to HRP, as a detection antibody in a cetuximab bridging ELISA for PK assay development together with HCA220 as the capture reagent.Protocol: PK bridging ELISA to measure total drug
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Cetuximab | HCA220 | E | 0.1 mg |  | |
| Human anti Cetuximab | HCA221 | E | 0.1 mg | | |
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Cetuximab | HCA220 | E | 0.1 mg | | |
| Human anti Cetuximab | HCA221 | E | 0.1 mg | | |
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody |
Product Specific References
References for Cetuximab antibody
- Bose, M.E. et al. (2016) Simulated Respiratory Secretion for Use in the Development of Influenza Diagnostic Assays.PLoS One. 11 (11): e0166800.
- Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibodyUS Patent Application US20170315118A1
Further Reading
- Gomez, D. et al. (2013) Cetuximab therapy in the treatment of metastatic colorectal cancer: the future frontier?Int J Surg. 11 (7): 507-13.
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