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AbD Serotec/GM-CSF antibody | CC305/0.1 mg/MCA1828GA

价格
面议
货号:MCA1828GA
浏览量:127
品牌:AbD Serotec
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Mouse anti Bovine GM-CSF antibody, clone CC305 recognizes bovine granulocyte macrophage colony-stimulating factor (GM-CSF) also known as CSF-2. Bovine GM-CSF is a 143 amino acid cytokine which stimulates proliferation and differentiation of hematopoietic precursors.Mouse anti Bovine GM-CSF antibody, clone CC305 has been used as a capture antibody in conjunction with Mouse anti Sheep GM-CSF, clone 3C2 as a detection reagent in a sandwich ELISA for the measurement of bovine GM-CSF produced by peripheral blood mononuclear cells in vitro (Kennedy et al. 2002).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised C57/BL mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P11052
Entrez Gene
CSF2
GO Terms
GO:0005125cytokine activity
GO:0005129granulocyte macrophage colony-stimulating factor receptor binding
GO:0005615extracellular space
GO:0006955immune response
GO:0008083growth factor activity
Regulatory
For research purposes only

Applications of GM-CSF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application NameVerifiedMin DilutionMax Dilution
ELISA5 ug/ml as coating antibody
Western Blotting
    Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    ELISA
    This product may be used as a capture antibody in a sandwich ELISA together with MCA1924 when conjugated to biotin as the detection reagent.

    Secondary Antibodies Available

    DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
    Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117AE WB 0.5 mgloader
    Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GAF IF 0.1 mgloader
    Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GAF WB 0.1 mgloader
    Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GAF IF WB 0.1 mgloader
    Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117FF 0.5 mgloader
    Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117PE WB 0.5 mgloader
    Goat anti Mouse IgG (Fc):FITCSTAR120FC F 1 mgloader
    Goat anti Mouse IgG (Fc):HRPSTAR120PE WB 1 mgloader
    Rabbit F(ab')2 anti Mouse IgG:RPESTAR12AF 1 mlloader
    Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13BC E P RE WB 1 mgloader
    Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR70F 0.5 mgloader
    Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR76F 1 mlloader
    Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR77C E P 0.5 mgloader
    Goat anti Mouse IgG/A/M:Alk. Phos.STAR87AC E WB 1 mgloader
    Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87PE 1 mgloader
    Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GAF IF WB 0.1 mgloader
    Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9BF 1 mgloader

    Product Specific References

    References for GM-CSF antibody

    1. Kennedy HE et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.Infect Immun. 70 (3): 1488-500.
    2. Boutet, P. et al. (2004) Delayed neutrophil apoptosis in bovine subclinical mastitis.J Dairy Sci. 87 (12): 4104-14.
    3. Bao, Z.J. et al. (2014) Recombinant bovine interferon-τ enhances in vitro development of bovine embryos by upregulating expression of connexin 43 and E-cadherin.J Dairy Sci. 97 (11): 6917-25.
    4. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal speciesVet Immunol Immunopathol. 88: 111-22.
    5. Davies, C.J. et al. (2004) Methods of minimizing immunological rejection of a nuclear transfer fetus.U.S. Patent Application: US 2004/0029825 A1

    Fluorescent Spectraviewer

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    • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
    • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
    • Select the lasers and filters you wish to include
    • Select combined or multi-laser view to visualize the spectra
    View more products with GM-CSF specificity
    AbD Serotec于2006年初由原来的Serotec、Design、Biogenesis和OBT四家公司合并而成,统一归英国著名的抗体公司MorphoSys旗下。合并后的AbD Serotec公司可以提供上万种抗体和免疫学试剂、单抗制备和多种规模的抗体生产和标记服务。最近AbD Serotec还建立了ISO 9001认证的生产机构,为其客户提供更高品质的免疫学产品和相关客户服务。提供各类用于免疫检测的CD抗体、诊断试剂、各种标记试剂。AbD (Antibodies Direct) 是世界领先的抗体技术公司之一MorphoSys公司的研究用抗体部门。AbD Serotec品牌创立于2006年初,用以将由Design, Biogenesis, Serotec, 和Oxford Biotechnology 提供的抗体产品和服务推向市场。这些抗体产品和服务包括10,000种抗体和免疫学试剂,从MorphoSys HuCAL(the Human Combinatorial Antibody Library)抗体库定制单克隆抗体,以及各种规模的抗体生产和轭合服务。    AbD Serotec 新近在英国牛津附近的Kidlington 开放了一个经ISO 9001认证的新生产设施,作为AbD Serotec 总部。AbD Serotec 以向其研究及工业领域客户提供最高质量的产品和服务而自豪。    产品线:     *Human Antibodies / Reagents      *Rodent Antibodies / Reagents     *Veterinary Antibodies / Reagents     *Cell Biology Antibodies / Reagents     *Cytokine Antibodies / Reagents     *Neurology Antibodies / Reagents     *Microbiology Antibodies / Reagents     *Paraffin-reactive Antibodies     *Alexa Fluor  Conjugates      *Secondary Antibodies / Reagents     *Antibody Labeling Kits     *Antibody Manufacturing Tools     *Protein Purification Kits     *Buffers / Reagents and Serums     *Bulk Antibodies / Reagents