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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Mouse anti Bovine GM-CSF antibody, clone CC305 recognizes bovine granulocyte macrophage colony-stimulating factor (GM-CSF) also known as CSF-2. Bovine GM-CSF is a 143 amino acid cytokine which stimulates proliferation and differentiation of hematopoietic precursors.Mouse anti Bovine GM-CSF antibody, clone CC305 has been used as a capture antibody in conjunction with Mouse anti Sheep GM-CSF, clone 3C2 as a detection reagent in a sandwich ELISA for the measurement of bovine GM-CSF produced by peripheral blood mononuclear cells in vitro (Kennedy et al. 2002).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised C57/BL mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P11052
- Entrez Gene
- CSF2
- GO Terms
- GO:0005125cytokine activity
- GO:0005129granulocyte macrophage colony-stimulating factor receptor binding
- GO:0005615extracellular space
- GO:0006955immune response
- GO:0008083growth factor activity
- Regulatory
- For research purposes only
Applications of GM-CSF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 5 ug/ml as coating antibody | ||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with MCA1924 when conjugated to biotin as the detection reagent.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E WB | 0.5 mg |  | |
| Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F IF | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) | STAR117D680GA | F WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg | | |
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | E WB | 0.5 mg | | |
| Goat anti Mouse IgG (Fc):FITC | STAR120F | C F | 1 mg | | |
| Goat anti Mouse IgG (Fc):HRP | STAR120P | E WB | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml | | |
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg | | |
| Goat anti Mouse IgG:FITC (Rat Adsorbed) | STAR70 | F | 0.5 mg | | |
| Goat anti Mouse IgG:RPE (Rat Adsorbed) | STAR76 | F | 1 ml | | |
| Goat anti Mouse IgG:HRP (Rat Adsorbed) | STAR77 | C E P | 0.5 mg | | |
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg | | |
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:Dylight®800 | STAR8D800GA | F IF WB | 0.1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg | |
Product Specific References
References for GM-CSF antibody
- Kennedy HE et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.Infect Immun. 70 (3): 1488-500.
- Boutet, P. et al. (2004) Delayed neutrophil apoptosis in bovine subclinical mastitis.J Dairy Sci. 87 (12): 4104-14.
- Bao, Z.J. et al. (2014) Recombinant bovine interferon-τ enhances in vitro development of bovine embryos by upregulating expression of connexin 43 and E-cadherin.J Dairy Sci. 97 (11): 6917-25.
- Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal speciesVet Immunol Immunopathol. 88: 111-22.
- Davies, C.J. et al. (2004) Methods of minimizing immunological rejection of a nuclear transfer fetus.U.S. Patent Application: US 2004/0029825 A1
Fluorescent Spectraviewer
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- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
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