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AbD Serotec/TNF Alpha antibody | CC328/0.25 mg/MCA2335B

价格
面议
货号:MCA2335B
浏览量:127
品牌:AbD Serotec
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Mouse anti Bovine TNF alpha antibody, clone CC328 recognizes bovine TNF alpha, a 17.5kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant bovine TNF alpha.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q06599
Entrez Gene
TNF
GO Terms
GO:0005125cytokine activity
GO:0005615extracellular space
Regulatory
For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application NameVerifiedMin DilutionMax Dilution
ELISA1ug/ml5ug/ml
    Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    Flow Cytometry
    Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    ELISA
    Biotinylated Mouse anti Bovine TNF alpha antibody, clone CC328 may be used as a detection antibody in a sandwich ELISA for bovine TNF alpha in combination with Mouse anti Bovine TNFα antibody, clone CC327 (MCA2334) as capture reagent. Recombinant Bovine TNFα (PBP005) may be used as a standard.

    Application Based External Images

    ELISA

    Product Specific References

    References for TNF Alpha antibody

    1. Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides.Vet. Immunol. Immunopathol. 95: 21-31.
    2. Whelan, A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide.Infect Immun. 71 (11): 6420-5.
    3. Guergnon J et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cells.Cell Microbiol. 5 (10): 709-16.
    4. Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumour necrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.Vet Immunol Immunopathol. 135 (3-4): 320-4.
    5. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
    6. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.Vet Res. 42: 49.
    7. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.J Comp Pathol. 150 (4): 434-48.
    8. Camejo, M.I. et al. (2014) TNF-alpha in bulls experimentally infected with Trypanosoma vivax: a pilot study.Vet Immunol Immunopathol. 162 (3-4): 192-7.

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    View more products with TNF ALPHA specificity
    AbD Serotec于2006年初由原来的Serotec、Design、Biogenesis和OBT四家公司合并而成,统一归英国著名的抗体公司MorphoSys旗下。合并后的AbD Serotec公司可以提供上万种抗体和免疫学试剂、单抗制备和多种规模的抗体生产和标记服务。最近AbD Serotec还建立了ISO 9001认证的生产机构,为其客户提供更高品质的免疫学产品和相关客户服务。提供各类用于免疫检测的CD抗体、诊断试剂、各种标记试剂。AbD (Antibodies Direct) 是世界领先的抗体技术公司之一MorphoSys公司的研究用抗体部门。AbD Serotec品牌创立于2006年初,用以将由Design, Biogenesis, Serotec, 和Oxford Biotechnology 提供的抗体产品和服务推向市场。这些抗体产品和服务包括10,000种抗体和免疫学试剂,从MorphoSys HuCAL(the Human Combinatorial Antibody Library)抗体库定制单克隆抗体,以及各种规模的抗体生产和轭合服务。    AbD Serotec 新近在英国牛津附近的Kidlington 开放了一个经ISO 9001认证的新生产设施,作为AbD Serotec 总部。AbD Serotec 以向其研究及工业领域客户提供最高质量的产品和服务而自豪。    产品线:     *Human Antibodies / Reagents      *Rodent Antibodies / Reagents     *Veterinary Antibodies / Reagents     *Cell Biology Antibodies / Reagents     *Cytokine Antibodies / Reagents     *Neurology Antibodies / Reagents     *Microbiology Antibodies / Reagents     *Paraffin-reactive Antibodies     *Alexa Fluor  Conjugates      *Secondary Antibodies / Reagents     *Antibody Labeling Kits     *Antibody Manufacturing Tools     *Protein Purification Kits     *Buffers / Reagents and Serums     *Bulk Antibodies / Reagents