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AbD Serotec/TNF Alpha antibody | CC327/100 Tests/1ml/MCA2334A488

价格
面议
货号:MCA2334A488
浏览量:127
品牌:AbD Serotec
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Mouse anti Bovine TNF alpha antibody, clone CC327 recognizes bovine TNF alpha, a 17.5 kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Fallow deer
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant bovine TNF alpha.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q06599
Entrez Gene
TNF
GO Terms
GO:0005125cytokine activity
GO:0005615extracellular space
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry 11/101/50
ELISA
Flow Cytometry 1
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
ELISA
This reagent may be used as a capture antibody in a sandwich ELISA for bovine TNF alpha in combination with MCA2335B as detection reagent. PBP005 may be used as a standard.

Secondary Antibodies Available

DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117AE WB 0.5 mgloader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GAF IF 0.1 mgloader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GAF WB 0.1 mgloader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GAF IF WB 0.1 mgloader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117FF 0.5 mgloader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117PE WB 0.5 mgloader
Goat anti Mouse IgG (Fc):FITCSTAR120FC F 1 mgloader
Goat anti Mouse IgG (Fc):HRPSTAR120PE WB 1 mgloader
Rabbit F(ab')2 anti Mouse IgG:RPESTAR12AF 1 mlloader
Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR70F 0.5 mgloader
Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR76F 1 mlloader
Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR77C E P 0.5 mgloader
Goat anti Mouse IgG/A/M:Alk. Phos.STAR87AC E WB 1 mgloader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87PE 1 mgloader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GAF IF WB 0.1 mgloader
Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9BF 1 mgloader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13BC E P RE WB 1 mgloader

Negative Isotype Controls Available

DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
Mouse IgG2b Negative Control:Alexa Fluor® 488MCA691A488F 100 Tests/1mlloader
Mouse IgG2b Negative ControlMCA691F 100 Testsloader

Application Based External Images

ELISA

Product Specific References

References for TNF Alpha antibody

  1. Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides.Vet Immunol Immunopathol. 95 (1-2): 21-31.
  2. Whelan, A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide. Infect Immun. 71 (11): 6420-5.
  3. Guergnon J et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaβ activation of Theileria parva-transformed B cells.Cell Microbiol. 5 (10): 709-16.
  4. Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumournecrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.Vet Immunol Immunopathol. 135: 320-4.
  5. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.J Dairy Sci. 93: 2458-70.
  6. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.Funct Integr Genomics. 10: 21-38.
  7. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
  8. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.Vet Res. 42: 49.
  9. Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.PLoS One. 6: e29194.
  10. García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).Vet Immunol Immunopathol. 149: 66-75.
  11. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.J Comp Pathol. 150 (4): 434-48.
  12. Luisa, C. et al. (2016) Evaluation of serum markers of blood redox homeostasis and inflammation in PCB naturally contaminated heifers undergoing decontaminationScience of The Total Environment. 542: 653-64.
  13. Maggioli, M.F. et al. (2016) Increased TNF-α/IFN-γ/IL-2 and Decreased TNF-α/IFN-γ Production by Central Memory T Cells Are Associated with Protective Responses against Bovine Tuberculosis Following BCG Vaccination.Front Immunol. 7: 421.
  14. Rutigliano, H.M. et al. (2016) Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.Biol Reprod. 95 (2): 39.
  15. Camejo, M.I. et al. (2014) TNF-alpha in bulls experimentally infected with Trypanosoma vivax: a pilot study.Vet Immunol Immunopathol. 162 (3-4): 192-7.
  16. Jolly A et al. (2016) Evidence of a pro-apoptotic effect of specific antibodies in a bovine macrophage model of infection with Mycobacterium avium subsp. paratuberculosis.Vet Immunol Immunopathol. 169: 47-53.

Fluorescent Spectraviewer

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View more products with TNF ALPHA specificity
AbD Serotec于2006年初由原来的Serotec、Design、Biogenesis和OBT四家公司合并而成,统一归英国著名的抗体公司MorphoSys旗下。合并后的AbD Serotec公司可以提供上万种抗体和免疫学试剂、单抗制备和多种规模的抗体生产和标记服务。最近AbD Serotec还建立了ISO 9001认证的生产机构,为其客户提供更高品质的免疫学产品和相关客户服务。提供各类用于免疫检测的CD抗体、诊断试剂、各种标记试剂。AbD (Antibodies Direct) 是世界领先的抗体技术公司之一MorphoSys公司的研究用抗体部门。AbD Serotec品牌创立于2006年初,用以将由Design, Biogenesis, Serotec, 和Oxford Biotechnology 提供的抗体产品和服务推向市场。这些抗体产品和服务包括10,000种抗体和免疫学试剂,从MorphoSys HuCAL(the Human Combinatorial Antibody Library)抗体库定制单克隆抗体,以及各种规模的抗体生产和轭合服务。    AbD Serotec 新近在英国牛津附近的Kidlington 开放了一个经ISO 9001认证的新生产设施,作为AbD Serotec 总部。AbD Serotec 以向其研究及工业领域客户提供最高质量的产品和服务而自豪。    产品线:     *Human Antibodies / Reagents      *Rodent Antibodies / Reagents     *Veterinary Antibodies / Reagents     *Cell Biology Antibodies / Reagents     *Cytokine Antibodies / Reagents     *Neurology Antibodies / Reagents     *Microbiology Antibodies / Reagents     *Paraffin-reactive Antibodies     *Alexa Fluor  Conjugates      *Secondary Antibodies / Reagents     *Antibody Labeling Kits     *Antibody Manufacturing Tools     *Protein Purification Kits     *Buffers / Reagents and Serums     *Bulk Antibodies / Reagents