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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Mouse anti Bovine interleukin-4, clone CC313 is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation (Heussler et al. 1992).IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with biotinylated Mouse anti Bovine IL-4 antibody, clone CC314 (MCA2372B) as a detection reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant bovine IL-4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells form immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P30367
- Entrez Gene
- IL4
- GO Terms
- GO:0005125cytokine activity
- GO:0005136interleukin-4 receptor binding
- GO:0005615extracellular space
- GO:0006955immune response
- GO:0008083growth factor activity
- GO:0030890positive regulation of B cell proliferation
- GO:0042113B cell activation
- GO:0043066negative regulation of apoptosis
- GO:0045348positive regulation of MHC class II biosynthetic process
- GO:0045671negative regulation of osteoclast differentiation
- GO:0045944positive regulation of transcription from RNA polymerase II promoter
- GO:0048295positive regulation of isotype switching to IgE isotypes
- GO:0048304positive regulation of isotype switching to IgG isotypes
- Regulatory
- For research purposes only
Applications of IL-4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 2ug/ml | 5ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- ELISA
- This reagent may be used as a capture antibody in sandwich ELISA assays for bovine IL-4 in combination with MCA2372B as a detection reagent, and recombinant bovine IL-4 (PBP006) as a standard.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E WB | 0.5 mg |  | |
| Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F IF | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) | STAR117D680GA | F WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | FIF WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg | | |
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | E WB | 0.5 mg | | |
| Goat anti Mouse IgG (Fc):FITC | STAR120F | C F | 1 mg | | |
| Goat anti Mouse IgG (Fc):HRP | STAR120P | E WB | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml | | |
| Goat anti Mouse IgG:FITC (Rat Adsorbed) | STAR70 | F | 0.5 mg | | |
| Goat anti Mouse IgG:RPE (Rat Adsorbed) | STAR76 | F | 1 ml | | |
| Goat anti Mouse IgG:HRP (Rat Adsorbed) | STAR77 | C E P | 0.5 mg | | |
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg | | |
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:Dylight®800 | STAR8D800GA | F IF WB | 0.1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg | | |
| Human anti Mouse IgG2a:FITC | HCA037F | F | 0.1 mg | | |
| Human anti Mouse IgG2a:HRP | HCA037P | E | 0.1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg | |
Application Based External Images
ELISA
Product Specific References
References for IL-4 antibody
- Hope, J.C. et al. (2005) Development of detection methods for ruminant interleukin (IL)-4.J Immunol Methods. 301 (1-2): 114-23.
- Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.J Immunol. 174 (11): 6702-15.
- Sipka, A. et al. (2013) Prednisolone and cefapirin act synergistically in resolving experimental Escherichia coli mastitisJ Dairy Sci. 96: 4406-18.
- Stabel, J.R. et al. (2013) Disparate Host Immunity to Mycobacterium avium subsp. paratuberculosis Antigens in Calves Inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii and M. bovis.Clin Vaccine Immunol. 20: 848-57.
- Stabel, J.R. et al. (2011) Mediation of host immune responses after immunization of neonatal calves with a heat-killed Mycobacterium avium subsp. paratuberculosis vaccine.Clin Vaccine Immunol. 18: 2079-89.
- Dacal, V. et al. (2009) Local and systemic cytokine responses during larval penetration in cattle experimentally infested with Hypoderma lineatum (Diptera: Oestridae).Vet Immunol Immunopathol. 131: 59-64.
- Olivier, M. et al. (2012) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.PLoS One. 7: e30430.
- Marinaro, M. et al. (2012) Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats.Res Vet Sci. 93: 662-7.
- Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production. Parasite Immunol. 31: 72-7.
- Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.J Comp Pathol. 150 (4): 434-48.
- Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print]
- Liravi, B. et al. (2015) Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge.BMC Pulm Med. 15: 101.
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