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AbD Serotec/IFN Gamma antibody | CC330/0.5 mg/MCA2112

价格
面议
货号:MCA2112
浏览量:127
品牌:AbD Serotec
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Mouse anti Bovine interferon γ antibody, clone CC330 recognizes bovine interferon gamma, also known as immune interferon. Interferon γ is a 143 amino acid ~20 kDa immunomodulatory cytokine secreted predominantly by T lymphocytes in response to infection by invading microorganisms. Interferon γ enhances cytotoxic activities of many cell types including macrophages, NK cells and T lymphocytes (Weiss et al. 2002).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant bovine IFN-gamma.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred. This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P07353
Entrez Gene
IFNG
GO Terms
GO:0009615response to virus
GO:0005125cytokine activity
GO:0005133interferon-gamma receptor binding
GO:0005615extracellular space
GO:0006955immune response
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application NameVerifiedMin DilutionMax Dilution
ELISA5ug/ml as capture antibody
ELISpot
Flow Cytometry 11/501/100
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
ELISA
Mouse anti Bovine interferon-γ antibody, clone CC330 may be used as a capture reagent in a sandwich ELISA in combination with Mouse anti Bovine interferon-γ antibody, clone CC302 (MCA1783B) as a detection reagent and recombinant bovine interferon-γ (PBP007A) as a protein standard.

Secondary Antibodies Available

DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117AE WB 0.5 mgloader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GAF IF 0.1 mgloader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GAF WB 0.1 mgloader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GAF IF WB 0.1 mgloader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117FF 0.5 mgloader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117PE WB 0.5 mgloader
Goat anti Mouse IgG (Fc):FITCSTAR120FC F 1 mgloader
Goat anti Mouse IgG (Fc):HRPSTAR120PE WB 1 mgloader
Rabbit F(ab')2 anti Mouse IgG:RPESTAR12AF 1 mlloader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13BC E P RE WB 1 mgloader
Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR70F 0.5 mgloader
Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR76F 1 mlloader
Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR77C E P 0.5 mgloader
Goat anti Mouse IgG/A/M:Alk. Phos.STAR87AC E WB 1 mgloader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87PE 1 mgloader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GAF IF WB 0.1 mgloader
Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9BF 1 mgloader

Negative Isotype Controls Available

DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
Mouse IgG1 Negative ControlMCA928F 100 Testsloader

Application Based External Images

ELISA

ELISpot

Product Specific References

References for IFN Gamma antibody

  1. Mwangi, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals.J Immunol. 169 (7): 3837-46.
  2. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
  3. Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.Vet Res. 41 (1): 4.
  4. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19.
  5. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.J Immunol. 185: 3313-25.
  6. Elhmouzi-Younes, J. et al. (2009) Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation.Vet Res. 2009 40: 54.
  7. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705.
  8. Begg, D.J. et al. (2009) Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne"s disease.J Vet Diagn Invest. 21: 187-96.
  9. Tourais-Esteves, I. et al. (2008) Neonatal goats display a stronger TH1-type cytokine response to TLR ligands than adultsDev Comp Immunol. 32: 1231-41.
  10. Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.J Immunol. 180: 5963-72.
  11. McLaughlin, K. et al. (2010) Hsp70 enhances presentation of FMDV antigen to bovine CD4+ T cells in vitro.Vet Res. 41: 36.
  12. Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production.Parasite Immunol. 31: 72-7.
  13. Baszler, T.V. et al. (2008) Bovine immune response to inoculation with Neospora caninum surface antigen SRS2 lipopeptides mimics immune response to infection with live parasites.Clin Vaccine Immunol. 15: 659-67.
  14. Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortus infections.PLoS One. 6:e21978.
  15. Appana, G. et al. (2013) Antemortem and postmortem examinations of the cattle calf naturally infected with Mycobacterium avium subsp. paratuberculosis.Eur J Microbiol and Immunol. 3: 241–51.
  16. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.BMC Vet Res. 10: 293.
  17. Köhler H et al. (2015) Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection.BMC Vet Res. 11 (1): 74.
  18. Bergmann, A. et al. (2015) In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis.PLoS One. 10 (4): e0123980.
  19. Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print]

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View more products with IFN GAMMA specificity
AbD Serotec于2006年初由原来的Serotec、Design、Biogenesis和OBT四家公司合并而成,统一归英国著名的抗体公司MorphoSys旗下。合并后的AbD Serotec公司可以提供上万种抗体和免疫学试剂、单抗制备和多种规模的抗体生产和标记服务。最近AbD Serotec还建立了ISO 9001认证的生产机构,为其客户提供更高品质的免疫学产品和相关客户服务。提供各类用于免疫检测的CD抗体、诊断试剂、各种标记试剂。AbD (Antibodies Direct) 是世界领先的抗体技术公司之一MorphoSys公司的研究用抗体部门。AbD Serotec品牌创立于2006年初,用以将由Design, Biogenesis, Serotec, 和Oxford Biotechnology 提供的抗体产品和服务推向市场。这些抗体产品和服务包括10,000种抗体和免疫学试剂,从MorphoSys HuCAL(the Human Combinatorial Antibody Library)抗体库定制单克隆抗体,以及各种规模的抗体生产和轭合服务。    AbD Serotec 新近在英国牛津附近的Kidlington 开放了一个经ISO 9001认证的新生产设施,作为AbD Serotec 总部。AbD Serotec 以向其研究及工业领域客户提供最高质量的产品和服务而自豪。    产品线:     *Human Antibodies / Reagents      *Rodent Antibodies / Reagents     *Veterinary Antibodies / Reagents     *Cell Biology Antibodies / Reagents     *Cytokine Antibodies / Reagents     *Neurology Antibodies / Reagents     *Microbiology Antibodies / Reagents     *Paraffin-reactive Antibodies     *Alexa Fluor  Conjugates      *Secondary Antibodies / Reagents     *Antibody Labeling Kits     *Antibody Manufacturing Tools     *Protein Purification Kits     *Buffers / Reagents and Serums     *Bulk Antibodies / Reagents