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商品描述
HA Antibody
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Product Description | This high affinity anti-HA antibody is developed using HA peptide (YPYDVPDYA) conjugated to KLH and affinity-purified with the HA peptide. The antibody is useful for detection, isolation and localization of the HA-tag proteins. |  Western-blot analysis of the HA-tag protein (50 kd) expressed cell lysate and affinity enriched by IP, with HA-tag antibody, HRP conjugates (ICP0751) 1: Molecular marker 2: 10 uL of HA-tag protein in lysate (original cell lysate) 3: 10 uL of His-tag protein in lysate (after IP depletion) 4: 10 uL of the HA-tag protein in 100 uL of the eluting buffer enriched with 25 ug of the anti-His tag (ICP0750) from 2 mL of the original lysate.  Western blot analysis of the cell extract with expression of HA-tag proteins (B-F) and without expression of HA (A). | |
Species | Rabbit | ||
Formulation | 500 µg/mL in PBS, 50% glycerol | ||
Immunogen | GST peptide EEHLYERDEGDKWR KLH conjugates | ||
Purification | Antigen-specific affinity chromatography | ||
Specificity | The antibody recognizes HA-tag proteins. | ||
Applications | ELISA (1:2500); WB (1:1000); IP (5 µg/sample) | ||
Scientific Description | Hemaglutinin peptide (HA) is frequently used as an affinity tag to facilitate the detection, isolation and localization of the HA-fusion proteins. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C.Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References | 1. Cancer Res.2010. 70: 10445.doi: 10.1158/0008-5472.CAN-10-3040 |
