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商品描述
Progesterone Antibody, HRP
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Product Description | This antibody was developed using progesterone 3-(O-carboxymethyl) oxime-KLH conjugates as the immunogen and affinity purified with progesterone-agarose affinity chromatography and competitively eluted by free progesterone. The antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. The antibody conjugates could be utilized for the detection and quantization of free progesterone. |  Direct competitive inhibition ELISA using immobilized anti-progesterone (3 µg/mL), and progesterone-labelled HRP(100 ng/mL). 50% inhibition occurred at 5 ng/mL of free progesterone under non-optimized conditions. | |
Formulation | 0.5 mg/ml in PBS, 10mg/mL BSA with 50% glycerol, or lyophilized powder. | ||
Specificity | The antibody recognizes free progesterone. | ||
Conjugation | Progesterone-3(O-Carboxymethyl) Oxime-NHS active ester, via an acylation reaction Progesterone/HRP molar ratio, approximately 3:1 | ||
Applications | Direct ELISA | ||
Scientific Description | Progesterone is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans and other species. Progesterone belongs to class of hormones called progestogens, and is the major naturally occurring human progestogen. | ||
Storage & Stability | Store product at –20ºC. Expiration date is six months from date of shipping if properly stored. |
