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商品描述
Ochratoxin A Antibody, HRP
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Product Description | This antibody was developed using ochratoxin A (OA)-KLH conjugates, linked via its free carboxyl group. The antibody was affinity purified with an OA-agarose affinity column. The purified antibody was chemically conjugated to HRP. It could be utilized for direct competitive detection and quantization of the food-borne mycotoxin, ochratoxin A. |  Direct competitive inhibition ELISA using immobilized anti-OA (1.5 µg/mL), and OA-labelled HRP (50 ng/mL). 50% inhibition occurred at 25 ng/mL of free ochratoxin A in non-optimized conditions. | |
Species | Rabbit | ||
Formulation | 0.25 mg/mL in stabilizing buffer | ||
Immunogen | Pure ochratoxin A linked to KLH, via the free carboxyl group of OA using the NHS-active ester method. | ||
Purification | Antigen-specific affinity chromatography | ||
Specificity | The antibody recognizes ochratoxin A (100%), C (400%), B (5%), and <1% of ochratoxin alpha. | ||
Applications | ELISA, affinity column enrichment Note: For better ELISA results, use a different method than the NHS-active ester method to prepare the paired antigen, such as the OA-OV with EDC method. | ||
Scientific Description | Ochratoxin A is a food borne mycotoxin which is carcinogenic and nephropathic to both humans and animals. Contamination with OA in food may be harmful to humans and animals. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date issix months from date of shipping if stored properly. | ||
Product Specific References |
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