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商品描述
Phospho-S6 Peptide Antibody
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Product Description | S6 peptide (KRRRLASLR) is a substrate for S6K and ILK. The affinity purified rabbit polyclonal anti-pS6 antibodies were developed with phosphorylated S6 (KRRRLApSLR) and absorbed with the non-phosphorylated S6 peptide (KRRRLASLR). This antibody has been utilized for the non-radioactive p70 S6K kinase assay. |
Species | Rabbit |
Formulation | 250 µg/mL in PBS, 50% glycerol |
Immunogen | Phosphorylated S6 peptide KRRRLApSLR-KLH conjugates |
Purification | Affinity purified with the phospho-peptide KRRRLApSLR on agarose and affinity absorbed with the non-phosphorylated S6 peptide |
Specificity | The antibody recognizes only the phosphorylated product of S6K. There are no cross reactionswith the non-phosphorylated peptide substrate. |
Tested | Human breast cancer tissues and mouse cells |
Applications | ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); Non-radioactive S6K kinase assay |
Scientific Description | The S6 substrate peptide (KRRRLASLR) is a substrate for p70 S6K and other kinases such as PKC, PKM and PKA. |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored. |
Product Specific References | 1. Pept. Res. 1991.4(3): 158-170. |
