品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
p53 Antibody, HRP
|
Product Description | This antibody was developed and affinity purified using a peptide based on the human p53 sequence (4-40 aa) . The antibody was conjugated to peroxidase (HRP) via reductive amination. It was tested to detect the human p53 specifically in human cell and tissue. Direct labeling of peroxidase of the primary antibody is believed to avoid the effect of the IgG (50-60 kDa) signal from the use of secondary antibodies. |  Western blot analysis of p53 in HeLa cell lysates (right) and CHO cell lysates (left) with anti-p53 HRP (ICP7115), 1 µg/mL | |
Species | Rabbit | ||
Formulation | 250 µg/mL in stabilized buffer with 50% glycerol | ||
Immunogen | Human p53 peptide (15 - 40 aa) KLH conjugates | ||
Purification | Peptide-affinity chromatography | ||
Conjugation | Reductive amination; HRP/Ab ratio - 2:1 | ||
Specificity | The antibody recognizes human p53 in cell and tissue. | ||
Applications | ELISA (1:2500); WB (1:2500) | ||
Scientific Description | P53 (tumour protein 53 or TP53) regulates the cell cycle by acting as a transcription factor. It suppresses tumours and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman". This is referring to its role in conserving stability by preventing genome mutation. P53 refers to its molecular mass (53 kDa) on SDS-PAGE. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C.Expiration date is six months from date of shipping if stored properly. |
