This antibody was developed using glutathione (GSH)-KLH conjugates and affinity purified with antigen-specific affinity chromatography. The antibody is conjugated to peroxidase (HRP) via reductive amination and could be utilized for detection and quantization of the reduced (GSH) and oxidized (GSSG) glutathione as well as glutathionylated proteins and peptides.

Rabbit

250 µg/mL in PBS with 50% glycerol

Glutathione-KLH conjugates linked through -S- bonds

Aldehyde-activated HRP via reductive amination

HRP/Ab ratio - 3:1

This pan-specific antibody recognizes both GSH and GSSG as well as glutathionylated proteins and peptides.

Quantitative ELISA; WB; IP (affinity chromatography); IF; IHC

Glutathione is a tripeptide. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione, an antioxidant, protects cells from toxins such as free radicals. Glutathione can also modify proteins via disulfide bonds. Redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes have been reported.

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.