The rabbit anti-acetyl lysine antibody is developed using acetylated KLH as the immunogen. It is affinity purified with acetyl lysine agarose, followed by conjugation with fluorescein isothiocyanate.

Rabbit

250 µg/mL in PBS (pH 7) with 50% glycerol

Acetylated KLH conjugates

Affinity chromatography with acetyl-lysine agarose

FITC:antibody = 10:1

For direct immunofluorescence experiments

Acetylation of lysine is an important, reversible modification. The activities of some proteins are controlled by acetylation of lysine. Histone acetyltransferases (HATs) acetylate the conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) that contain lysine residues. Histone deacetylases (HDACs) remove the acetyl group from the same residue. ^[1] Acetylation/deacetylation of histones results in cell signalling processes that include gene activity, cell growth, differentiation and apoptosis. ^[2] In cancer and polyglutamine diseases, the regulation of protein acetylation/deacetylation is impaired (7). Numerous anti-cancer drugs target HDACs. ^[3]

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.