NanoHelix Uracil-DNA-glycosylase(UDG), also known as UNG, is an engineered E. coli enzyme catalyzing the hydrolysis of N-glcosylic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. This enzyme has been engineered to be more susceptible to high-temperature and inactivated easily during the dUTP containing PCR or real-time PCR. The wild-type of E. coli UDG is resistant to thermal inactivation. For PCR diagnosis, UDG can be used for efficient removal of carryover contaminated uracil-containing DNA. 

Application 

• Elimination carryover contamination
• Genotyping and Molecular Diagnosis  

Contents 

• Uracil-DNA glycosylase (1 unit/ul) 

Quality control

Uracil-DNA glycosylase is evaluated by the removal of PCR product using dUTP with 1 unit of this enzyme.