HelixChem™ ShinyStar gel stain is a safe nucleic acid stain, an alternative to the traditional ethidium bromid(EtBr) stain for detecting nucleic acid on agarose gels. It emits green fluorescence when bound to DNA or RNA under UV light.
Visualization of DNA and RNA bands on an agarose gel electrophoresis.
Figure 1. Gel analysis of serially diluted PCR products and 1 kb DNA ladder using HelixChem™ ShinyStar gel stain and EtBr.
Figure 2. Gel analysis of different size of PCR products using HelixChem™ ShinyStar gel stain and EtBr. Each PCR product was synthesized using HelixAmp™ Speed-Pfu polymerase. M: HelixRuler™ 1kb+ (II) DNA ladder.
Figure 3. Gel analysis of serially diluted RNA using HelixChem™ ShinyStar gel stain and EtBr. Total RNA was isolated from Plant’ leaf using PureHelix™ Total RNA Purification Kit. M: HelixRuler™ 1kb+ (II) DNA ladder.
Storage and Stability
- Store at 4℃ and stable for more than 12 month.
- HelixChem™ ShinyStar gel stain is light sensitive and should be stored in a dark.
Application
- Visualization of DNA and RNA bands on an agarose gel electrophoresis.
Safety tests
Table 1. HelixChem™ ShinyStar gel stain safety test results. The dye is non-cytotoxic and non-mutagenic at working concentrations used in gel staining.
NanoHelix的Bst DNA聚合酶,大片段是嗜热脂肪芽孢杆菌 DNA聚合酶蛋白的一部分,具有5'→3'聚合酶活性,但缺乏5'→3'核酸外切酶活性。Bst DNA聚合酶,大片段作为重组体从大肠杆菌菌株中纯化。 应用 回路介导的等温扩增(LAMP)DNA链置换扩增全基因组扩增