HelixAmp™ Bst DNA polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5’ → 3’ polymerase activity, but lacks 5’ → 3’ exonuclease activity. Bst DNA polymerase, Large Fragment is prepared from an E. coli strain containing the Bacillus stearothermophilus DNA polymerase gene, lacking the 5’ → 3’ exonuclease domain. 

Application 

• Isothermal amplification (LAMP)
• DNA sequencing through high GC resions
• Rapid Sequencing from nanogram amounts of DNA template 

Contents 

• Bst DNA polymerase, Large Fragment (8 units/μl)