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NanoHelix/qRT-PCR Kit [v6] (UDG System), Bulk/Bulk/BCVQR6

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货号:BCVQR6
浏览量:127
品牌:NanoHelix
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商品描述

qRT-PCR Kit [v6] (UDG System), Bulk

 

Products

Cat.No.ProductFeatureSize
BCVQR6RealHelix™ qRT-PCR Kit [v6] (UDG System)+ UDG SYSTEM*Bulk
  • Description
  • Data
  • Specification
  • Documents

qRT-PCR Kit [v6] (UDG System) is a quantitative RT-PCR reagent suitable for molecular diagnosis of SARS-CoV-2 causing the new Coronavirus infectious disease (COVID-19). The kit performance was confirmed using the materials and methods approved by WHO on 17 Jan, 2020. The Enzyme Mix contains in this kit, is an optimized blend of Reverse Transcriptase, antibody-coupled hot-start Taq polymerase (Ab+Taq polymerase), RNase inhibitor protein and a thermolabile UDG. These components help ensure sensitivity, accuracy, and specificity of PCR performance for the COVID-19 molecular diagnosis. In particular, carryover contaminated PCR products could be removed by the applied UDG system.

 

Application 

  • Quantification of target RNA by real-time RT-PCR

Character

  • Use the antibody-coupled Hot-Start Taq (Ab+Taq)
  • Pre-denaturation time in PCR steps = < 5 min
NanoHelix的Bst DNA聚合酶,大片段是嗜热脂肪芽孢杆菌 DNA聚合酶蛋白的一部分,具有5'→3'聚合酶活性,但缺乏5'→3'核酸外切酶活性。Bst DNA聚合酶,大片段作为重组体从大肠杆菌菌株中纯化。    应用 回路介导的等温扩增(LAMP)DNA链置换扩增全基因组扩增