Hot-Taq polymerase glycerol-free is a lyophilization-compatible version of Hot-Taq polymerase, a chemically modified enzyme, and quite suitable for high-specific hot-start PCR, real-time PCR and multiplex PCR. The 10x reaction buffer supplied together contains pH-buffering agent, salts, magnesium, and dNTPs.

Application 

• Hot-start PCR, Multiplex PCR, Real-time PCR
• High specific and sensitive PCR
• Amplification of complex DNA and cDNA
• PCR for molecular diagnostics
• Manufacture of dried amplification mixtures

Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activity

Enzyme activation: 15 min at +95°C

Storage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Tween-20

DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr) 

RNase contamination test: Not detectable (Incubation with 40U enzyme and Human total RNA at 37°C, 1hr)

Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)

Stability: 24 months at -20°C