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蚂蚁淘在线 / 品牌中心 / metkinen / metkinen chemistry/Metkinen Universal Solid Support III/103-00

metkinen chemistry/Metkinen Universal Solid Support III/103-00

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货号：103-00
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Metkinen Universal Solid Support III
Metkinen Chemistry - reagents for DNA and RNA synthesisMetkinen Chemistry - Reagents for RNA and DNA synthesis.
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Metkinen Universal Solid Support III

The universal support strategy offers the following clear advantages: eliminates the possibility of errors in parallel synthesis applications where up to 384 wells may contain different supports; eliminates the need for at least four supports for DNA synthesis and four for RNA synthesis; simplifies the preparation of oligonucleotides with modified or unusual nucleosides at the 3’-terminus. Like our initial version of Universal Solid supports - USII, the new type of supports - Metkinen Universal Solid Support III would be appropriate for the production of DNA oligos, long and short, as well as those requiring mild deprotection. It is compatible with the synthesis of RNA, (including siRNA) as well as virtually any oligonucleotide analogs (2’-F-RNA, 2’-OMe-RNA, LNA, oligonucleotide N3’→P5’phosphoramidates, etc.). The reagent used for the cleavage/dephosphorylation step is commercially available and the procedures described are fully compatible with high-throughput Synthesis. The difference lies in the higher stability of Metkinen Universal Solid Support III than that of USII upon prolong storage. In addition, the preparation of the new support appears to be more consistent and reliable. Metkinen Universal Solid Support III is subject to proprietary rights of Glen Research Corporation and is synthesized and sold under the following licensed patents: US Patent No.: 6,770,754 and European Patent No.: 1404695. The new carbomoylation chemistry, resulting in the stable urea fragment, bridging the Universal linker and aminoalkylated solid phase, is subject to proprietary rights of Metkinen Chemistry (U.S. Patent Application Serial No. 60/854,721; International Patent Application No. PCT/ FI2007/050575). Metkinen Universal Solid Support III for synthesis of DNA, RNA & any type of modified oligonucleotides. Catalogue number: 103-00Description: Chemically modified MacropourousAminomethyl polystyrene. White tooff-white powder.Storage of dry compound: 1 year at +20ºCLoading: 20-80 µmol/g g loadings is available.Please enquire for custom loading.Oligo synthesis on USIII: Perform oligonucleotideassembly, using standard protocols, recommendedby your synthesizer manufacturer. Uponthe completion of synthesis wash the oligonucleotidebound support with pure acetonitrile. Donot perform any washing steps with solvents,containing basic reagents (diethylamine, triethylamine,dimethylamine, etc.) and water!Cleavage: Cleave the oligo from the supportusing 3.5N - 4.5N ammonia in methanol (dilutecold 7N ammonia in methanol, Aldrich Cat. No499145-100ML, with cold anhydrous methanol)at room temperature for 30 minutes. Donot use aqueous ammonium hydroxide and/ormixtures of ammonium hydroxide and methanolfor cleavage!Deprotection AFTER Cleavage:Standard: After Cleavage, add 1 volume of 3.5N- 4.5N ammonia in methanol, seal and deprotectfor 8-15 hours at 60 °C for removal of theprotecting groups on the nucleobases.orAlternatively: add 1 volume of 30% ammoniumhydroxide, seal and deprotect using the conditionsappropriate for removal of the protectinggroups on the nucleobases (e.g. at 55 °C for 5 hours).Price and Ordering Information
Copyright © 1996 - , Metkinen Chemistry, Microkatu 1,Microtower osa M,70210 Kuopio,Finland,Phone: +358 40 736 4885, +358 40 554 2218.E-mail: info@metkinenchemistry.com
metkinen chemistry嘌呤3'-氨基-3'-脱氧核苷通过生物转化合成3'-氨基-3'脱氧核苷，并用作制备相应的3'-氨基-3'-脱氧核苷-5'-CE亚磷酰胺的起始化合物。后者是组装RNA寡核苷酸N3'-> P5'氨基磷酸酯的基础。