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蚂蚁淘在线 / 品牌中心 / metkinen / metkinen chemistry/Chemical Phosphorylation Reagent II/103-10

metkinen chemistry/Chemical Phosphorylation Reagent II/103-10

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￥80000.00
货号：103-10
浏览量：127
品牌：metkinen
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Chemical Phosphorylation Reagent
Metkinen Chemistry - reagents for DNA and RNA synthesisMetkinen Chemistry - Reagents for RNA and DNA synthesis.
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Chemical Phosphorylation Reagent II

Chemical Phosphorylation Reagent (CPR II) contains a DMT group which can be left on the oligonucleotideand used for rapid purification of oligonucleotide 5"-phosphates by the popular DMTr-on technique, whichemploys disposable RP cartridges or “Trityl-on” RP HPLC purification. The DMTr group is removedwith aqueous acid (e.g., 2%TFA in the case of Cartridge Purification) and the remaining linker is theneliminated after brief treatment with aqueous ammonium hydroxide (12 -15% ammonium hydroxide atroom temperature for 15 minutes) to yield the 5"-phosphate.[3-(4,4"-Dimethoxytrityloxy)-2,2-dicarboxyethyl]propyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramiditeCatalogue number: 103-10Description: amorphous colorless glassStorage of dry compound: 1 year at -20ºCCoupling Conditions: 6 minute coupling time. Omit the capping step after the addition of this reagent!Price and Ordering Information
Copyright © 1996 - , Metkinen Chemistry, Microkatu 1,Microtower osa M,70210 Kuopio,Finland,Phone: +358 40 736 4885, +358 40 554 2218.E-mail: info@metkinenchemistry.com
metkinen chemistry嘌呤3'-氨基-3'-脱氧核苷通过生物转化合成3'-氨基-3'脱氧核苷，并用作制备相应的3'-氨基-3'-脱氧核苷-5'-CE亚磷酰胺的起始化合物。后者是组装RNA寡核苷酸N3'-> P5'氨基磷酸酯的基础。