| | | 6-SIMA phosphoramidite[(3,6-dipivaloyl-2,7-diphenyl-4,7-dichlorofluoresceinyl)-6-carboxamidohexyl]-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramiditeCatalogue number: 303-55SIMA is a full spectral analog of HEX in all fluorescent applications, but has significantly improved stability. It has a higher quantum yield and molecular extinction than HEX. SIMA is also more versatile in oligo synthesis especially due to improved stability during deprotection and simple purification of labeled oligos Formula:: C58H64Cl2N3O10PMolecular Weight: 1065.0Purity: > 95%Diluent: Anhydrous AcetonitrileCoupling: No changes needed from standard method recommended by synthesizer manufacturer.Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.SIMA labeled oligos are stable at 55°C in ammonium hydroxide (up to 6-8 hours) and canbe deprotected with AMA.Storage: Freezer storage, -10 to -30°C, dry.Stability in Solution: 2-3 days, <90% efficient after 4 daysStore labeled oligo in the dark, either dry or in a neutral aqueous media at -20°C.Do not store crude fluorescently labeled oligonucleotides in ammonia.Price and Ordering Information | |
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