- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
Data/Specifications
Ig Isotype: Rat IgG1k
Specifcity:Keyhole limpet hemocyanin (KLH)
Form:This product supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2.
Storage:Store at 2 – 8 °C
Literature/Support
PDF Insert
How To Use
Applications:
- Used for Flow cytometry.
- Used for Enzyme-Linked–Immunosorbent-Assay (ELISA): recommended dilution of 1 ug/mL
- Used for Flourescent-Linked-Immunosorbent-Assay (FLISA).
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs
