- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
Data/Specifications
Ig Isotype: Mouse IgG1k
Specifcity:T-2 mycotoxin
Form:The low endotoxin, azide-free antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
Storage:Store at 2 – 8 °C. Aliquot and store at or below -20°C.
Literature/Support
PDF Insert
How To Use
Applications:
- Used for Flow cytometry
- Used for Enzyme-Linked –Immunosorbent-Assay (ELISA)
- Used for Flourescent-Linked-Immunosorbent-Assay (ELISA)
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs
