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Klen Taq/OmniTaq 3/125 ul (500 X 25 ul rxns)/303

价格
¥6000.00
货号:303
浏览量:127
品牌:Klen Taq
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商品描述

OmniTaq 3 DNA polymerase is a mutant of Taq polymerase that makes the enzyme resistant to the inhibitory effects of blood, soil, and more.  It remains functional in up to 40% whole blood, especially in the presence of our enhancer products. OmniTaq 3 is suitable for direct amplification of samples containing plant tissues and feces. It also works in some concentrations of crude soil extract or inhibitory food matrices where other commercial enzymes fail. 

Vanderbilt Researchers detect SNP markers for antimalarial resistance using OmniTaq 3.

Catalog Number
303
MSDS
File
SDS- OmniTaq 3
Amount
125 ul (500 X 25 ul rxns)

Direct Blood PCR Performance of Inhibition-resistant OmniTaq 3

New Taq mutant dramatically outperforms the wild type Taqand remains functional in at least up to 40% blood.

A 1 kb target was amplified directly from human blood, present at 5-40% in 50 ul reaction, with 0.5 ul “1X” OmniTaq 3 or similar enzyme strength (1 μl/10U) of plain Taq (NEB). No PCR enhancer was used.  Control reactions (C) contained no blood but 10 ng human DNA.                                                             

Results: The new Taq mutant dramatically outperforms the wild type Taq  and remains functional in at least up to 40% blood. This enzyme strength overamplified and smeared the OT3 control.  Up to 5x less enzyme is needed for purified DNA (results not shown).          

Klen Taq的OmniTaq酶具有所需的5'核酸酶活性,因此可与Taqman分析兼容。Klentaq产品与该测定法不兼容。