BCIP/NBT
Cat. No. 4410
BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/ Nitro Blue Tetrazolium) liquid ready-to-use substrate is a highly active and stable formulation utilized for colorimetric detection of Alkaline Phosphatase (AP) activity in membrane assays. Positive reactions form an intense blue/purple precipitate at the site of the reaction. The color develops when AP catalyzes the dephosphorylation of BCIP and converts NBT to insoluble blue/purple NBT formazan. The intense blue/purple precipitate is very stable and resists fading when exposed to light.
The product is available in bulk quantities and as customized solutions.
Specifications
- Available sizes:100 mL, 500 mL, 1 L, bulk
- Format:Ready-to-use (RTU)
- Storage:2-8⁰C in the dark
- Stability:4 years
- Development time:5-30 minutes