TMB PLUS2® (& prestained)
Cat. No. 4395 / 4445
TMB (3, 3", 5, 5"-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).
TMB PLUS2® characteristics are:
- Medium sensitivity
- Very low background
- Aqueous based – no organic solvents (REACH Annex XVII compliant)
- Low lot-to-lot variation
For traceable pipetting, we recommend using pink colored TMB PLUS2® Prestained (cat. no. 4445). The pink indicator disappears after addition of the stop solution.
The product is available in bulk quantities and as customized solutions.
Specifications
- Available sizes:100 mL, 500 mL, 1 L, bulk
- Format:Ready-to-use (RTU)
- Storage:2-8⁰C in the dark
- Stability:4 years
- Development time:5-30 minutes
- Stop solution:0.2 M H₂SO₄
Downloads
Download your LOT specific Certificate of Analysis (CoA)
ECO-TEK
- Free of Toxic Preservatives
- Free of Bovine Serum Albumin
- Free of Harmful Organic Solvents – No NMP
- Free of Hazardous Stop Solutions