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蚂蚁淘在线 / 品牌中心 / Cellagen / Cellagentech/Repro-ThiPSC | Enhance reprogramming efficiency cocktail/K8447-500/0.5mL of 1000x
商品描述
ProductDescription
Repro-ThiPSC(i.e.SMC4inreference1)isacocktailoffoursmallmoleculesofspecificsignalingpathwayinhibitors.ItcanbeaddeddirectlyinmediatoincreasereprogrammingefficiencyofsomaticcellstoiPSCs,andtoimproveexpansionandmaintenanceofhiPSCsunderbothwithfeedercellsandfeeder-freeconditions.Itcanbeusedincombinationwithanyofthewidelyusedreprogramminggeneexpressionsystems(e.g.retro/lenti-viral,episomal,mRNA,orproteins)toenhancethereprogrammingsomaticcellstoiPScells.Thisprocessisrobust,efficientandcanbeeasilyscaled-up1-3.
Technicalinformation:
| CocktailFormulation: 1000xDMSOstocksolution | 5mMofThiazovivin(ROCKinhibitor), 0.4mMofPD325901(MEKinhibitor), 2mMofSB431542(TGF-ß1inhibitor),and 1mMofCHIR99021(GSK3ßinhibitor) | |
| Purity: | >98%(foreachcompound) |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storage:StoreDMSOsolutionat-20oC.
Usage:DiluteRepro-ThiPSCstocksolution(1000x)at1:1000intobasiciPSCinductionmediatomake1xRepro-ThiPSCmedia.
Reference:
| 1. | ValamehrB,etal.Anovelplatformtoenablethehigh-throughputderivationandcharacterizationoffeeder-freehumaniPSCs.ScientificReports.2012.2:213. |
| 2. | LiW,etal.Generationofhuman-inducedpluripotentstemcellsintheabsenceofexogenousSox2.StemCells.2009.27(12):2992-3000. |
| 3. | KawamataM,etal.Generationofgeneticallymodifiedratsfromembryonicstemcells.PNAS.2010.107,14223-14228. |
Cellagen的ChamQ SYBR qPCR预混液（无ROX）| Q321产品描述ChamQSYBR®qPCR预混液（Vazyme，＃Q321）由香槟Taq DNA聚合酶（Vazyme，＃P122）通过抗体修饰的热启动激活技术保护，是专为基于SYBR Green I的定量PCR（qPCR）设计的。ChamQSYBR®qPCR Master Mix的优化缓冲液系统中的独特因素（即促进特异性的Exactor）大大提高了其灵敏度和特异性。该混合物以2倍反应浓度制备，可直接用于鲁棒性低模板qPCR，具有高灵敏度，特异性和可靠性。优点特异性+敏感性的最佳组合。独特的抗体修饰的热启动taq，具有独特的特异性促进因子。Mg2 +和染料的最佳浓度。
