ProductDescription
Datahasshowndirecttransdifferentiationbetweendifferentgermlayerswhentransfectedwithacocktailofdefinedtranscriptionfactorgenes1,3.Asupplementofthissmallmoleculecocktailincellculturemediasimplifiestheprocessandincreasestheproductivityofneuralandneuronalcellgeneration.
Repro-nLSCisacocktailofthreesmallmoleculeinhibitors.ItincludesLDN193189,SB431542andCHIR99021.UsingthecombinationofsynergisticSMADinhibitionthroughBMPandTGF-betapathwaystogetherwithspecificGSK-3betainhibition,arecentstudyhaspresentedthatpostnatalhumanfibroblasts(HPF),transducedwithAscl1andNgn2,efficientlyconvertedintofunctionalneuron-likecellswithyieldsupto>200%andneuronalpuritiesupto>80%.Inadditiontotheconversionofmesenchymalcellstoinducedneurons(iNs),thismethodhasalsobeentestedintheconversionofothersomaticcelltypes(eg.Cordblood-derivedstemcells)toiNs3.
Technicalinformation:
| CocktailFormulation: 1000xDMSOstocksolution | 0.5mMofLDN193189(BMPinhibitor), 10mMofSB431542(TGF-ß1inhibitor),and 2mMofCHIR99021(GSK3ßinhibitor) | |
| Purity: | >98%(foreachcompound) |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storage:StoreDMSOsolutionat-20oC.
Usage:DiluteRepro-nLSCstocksolution(1000x)at1:1000intoyourreprogrammingmediatoinitiateneuronalconversion.
Reference:
| 1. | VierbuchenT,etal.Directconversionoffibroblaststofunctionalneuronsbydefinedfactors.Nature.2010.463(7284):1035-41. |
| 2. | ChambersSM,etal.HighlyefficientneuralconversionofhumanESandiPScellsbydualinhibitionofSMADsignaling.NatBiotechnol.200927(3):275-80. |
| 3. | LadewigJ.,etal.Smallmoleculesenablehighlyefficientneuronalconversionofhumanfibroblasts.NatMethods.2012.9(6):575-8. |
Cellagen的ChamQ SYBR qPCR预混液（无ROX）| Q321产品描述ChamQSYBR®qPCR预混液（Vazyme，＃Q321）由香槟Taq DNA聚合酶（Vazyme，＃P122）通过抗体修饰的热启动激活技术保护，是专为基于SYBR Green I的定量PCR（qPCR）设计的。ChamQSYBR®qPCR Master Mix的优化缓冲液系统中的独特因素（即促进特异性的Exactor）大大提高了其灵敏度和特异性。该混合物以2倍反应浓度制备，可直接用于鲁棒性低模板qPCR，具有高灵敏度，特异性和可靠性。优点特异性+敏感性的最佳组合。独特的抗体修饰的热启动taq，具有独特的特异性促进因子。Mg2 +和染料的最佳浓度。
