Y-27632 is widely used as a specific inhibitor of the Rho-associated coiled-coil forming protein serine/threonine kinase (ROCK) family. It has been found to prevent dissociation-induced apoptosis in human embryonic stem cells and enhance the survival and cloning efficiency of dissociated hES cells without affecting their self-renewal properties or pluripotency. Y-27632 can also enhance survival during the transplantation of ES cell-derived neural precursors.

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Technical information:

Chemical Formula:		C14H21N3O·2HCl
CAS #:		146986-50-7
Molecular Weight:		329.27
Purity:		>98%
Appearance:		White solid
Chemical Name:		(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide
Solubility:		Up to 100 mM in DMSO

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Shipping Condition: The product is shipped in a glass vial at ambient temperature.Storage: For longer shelf life, store solid powder at 4^oC desiccated, or store DMSO solution at -20^oC.

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Reference:

1.	Ishizaki, T. et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol (2000) 57: 976-983.
2.	Koyanagi et al. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J. Neurosci Res (2008) 86: 270-280.
3.	Watanabe et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotech (2007) 25: 681-686.

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Other Information:

Product Specification (pdf)MSDS (pdf)

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