ProductDescription
CX-4945isanorally-available,ATP-competitive,pyridoquinoline-basedinhibitorofCK2awithanIC50potencyof1nMandKiof0.38nM.[1,2]Inapanelof238kinasesat500nM,only7inhibitedCK2>90%[2]TreatmentofBT474andBxPC-3cellswithCX-4945ledtorapiddephosphorylationofCK2,Akt(S129),accompaniedbyreductioninphsophorylationatT308andS473ofAkt.SincenoreductioninPTENphosphorylationatCK2"sS370/S380,itissurmisedthatCX-4945suppressesPi3K/AktsignalingbydirectlyblockingphosphorylationofAktatAktatS129byCK2,ratherthanthroughactivationofPTEN.[2]
Additionally,CX-4945inhibitshypoxia-drivenhypoxiainducIBLefactor(HIF-1a)activityandpreventsdownregulationofpVHLandp53.CX-4945alsoexertsaregulatoryeffectonthesecretionofIL-6ininflammatorybreastcancer.[3]
CX-4945mayalsobeapotentialsensitizerforresistantcellstoconventionalchemotherapeuticagentssuchasgemcitABIneandcisplatin.[4]
Technicalinformation:
| ChemicalFormula: | C19H12ClN3O2 | |
| CAS#: | 1009820-21-6 | |
| MolecularWeight: | 349.77 | |
| Purity: | >99% | |
| Appearance: | Yellow | |
| ChemicalName: | 5-(3-chlorophenylamino)benzo[c][2,6]naphthyridine-8-carboxylicacid | |
| Solubility: | Upto100mMinDMSO | |
| Synonyms: | CX-4945,CX4945,CX4945,Silmitasertib |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storagecondition:Forlongershelflife,storesolidpowderat4oCdesiccated,orstoreDMSOsolutionat-20oC.
Reference:
| 1. | Pierreetal.,Pre-clinicalcharacterizationofCX-4945,apotentandselectivesmallmoleculeinhibitorofCK2forthetreatmentofcancer.Mol.Cell.Biochem.,2011,356,37-43.PubmedID:21755459 |
| 2. | Siddiqui-Jainetal.,CX-4945,anorallybioavailableselectiveinhibitorofproteinkinaseCK2,inhibitsprosurvivalandangiogenicsignalingandexhibitsantitumorefficacy.CancerRes.2010,70,10288-10298.PubmedID:21159648 |
| 3. | Kimetal.,DruggabilityoftheCK2inhibitorCX-4945asananticancerdrugandbeyond..Arch.Pharm.Res.2012,35(8),1293-1296.PubmedID:22941473 |
| 4. | Zaninetal.,EffectsoftheCK2inhibitorsCX-4945andCX-5011ondrug-resistantcells..PLosONE,2012,7(11),e49193.PubmedID:21159648 |
Cellagen的ChamQ SYBR qPCR预混液（无ROX）| Q321产品描述ChamQSYBR®qPCR预混液（Vazyme，＃Q321）由香槟Taq DNA聚合酶（Vazyme，＃P122）通过抗体修饰的热启动激活技术保护，是专为基于SYBR Green I的定量PCR（qPCR）设计的。ChamQSYBR®qPCR Master Mix的优化缓冲液系统中的独特因素（即促进特异性的Exactor）大大提高了其灵敏度和特异性。该混合物以2倍反应浓度制备，可直接用于鲁棒性低模板qPCR，具有高灵敏度，特异性和可靠性。优点特异性+敏感性的最佳组合。独特的抗体修饰的热启动taq，具有独特的特异性促进因子。Mg2 +和染料的最佳浓度。
