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inspiralis/Buffers/1ml 5X Assay Buffer/SPB001

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¥840.00
货号:SPB001
浏览量:24
品牌:inspiralis
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Buffers

Technical Documents

Cat No.ProductPriceQuantity
B0001E.coli gyrase assay buffer 1ml5X Assay Buffer£42
B0002E.coli gyrase dilution buffer1ml Dilution Buffer£42
TR001E.coli topo IV relaxation assay buffer 1ml5X Assay Buffer£42
TD003E.coli topo IV decatenation assay buffer1ml 5X Assay buffer£42
TD002E.coli topo IV dilution buffer1ml Dilution Buffer£42
SAB001S.aureus gyrase assay buffer 1ml5X Assay Buffer£42
SAB002S.aureus gyrase dilution buffer1ml Dilution Buffer£42
SATR001S.aureus topo IV assay buffer 1ml5X Assay Buffer£42
SATD002S.aureus topo IV dilution buffer1ml Dilution Buffer£42
MTB001M.tuberculosis gyrase assay buffer 1ml5X Assay Buffer£42
MTB002M.tuberculosis gyrase dilution buffer1ml Dilution Buffer£42
PAB001P.aeruginosa gyrase assay buffer 1ml5X Assay Buffer£42
PAB002P.aeruginosa gyrase dilution buffer1ml Dilution Buffer£42
PATB001P.aeruginosa topo IV assay buffer 1ml5X Assay Buffer£42
PATD001P.aeruginosa topo IV dilution buffer 1mlDilution Buffer£42
SPB001S.pneumoniae gyrase assay buffer 1ml5X Assay Buffer£42
SPB002S.pneumoniae gyrase dilution buffer 1mlDilution Buffer£42
SPT001S.pneumoniae topo IV assay buffer 1ml5X Assay Buffer£42
SPT002S.pneumoniae topo IV dilution buffer1mlDilution Buffer£42
ABT4001A.baumannii topo IV assay buffer1ml5X Assay Buffer£42
ABT4002A.baumannii topo IV dilution buffer1mlDilution Buffer£42
ABB001A.baumannii gyrase assay buffer1ml5X Assay Buffer£42
ABB002A.baumannii gyrase dilution buffer1mlDilution Buffer£42
WGB001Wheatgerm topo I assay buffer1ml2X Assay Buffer£42
WGB002Wheatgerm topo I dilution buffer1mlDilution Buffer£42
HTA101Human topo I assay buffer1ml 10X Assay Buffer£42
HTD102Human topo I dilution buffer1mlDilution Buffer£42
HTA202Human topo II assay buffer1ml 10X Assay Buffer£42
HTD202Human topo II dilution buffer1ml Dilution Buffer£42
ATPB00130mM ATP1ml 30mM ATP£42
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inspiralis高/中通量拓扑异构酶测定John Innes中心的A. Maxwell教授和Inspiralis员工在实验室中开发了一种基于高通量微量滴定板的测定法。基于这样的事实,即负超螺旋质粒比松弛质粒更容易形成分子间三链体DNA。该测定法克服了基于凝胶的测定法的一些问题,这些问题很费时,因此固有地是低通量的。Inspiralis能够提供此测定方法,作为筛选化合物对拓扑异构酶(包括促旋酶,topo IV和人topos I和II)的抑制活性的手段。该测定法适用于改变DNA超螺旋的其他酶,例如限制酶。该测定与常规基于凝胶的测定比较的一些结果如下所示。该测定法对化合物的筛选特别有用,也可用于测定IC 50  值。在以下给出的实施例中,比较了基于板和凝胶的测定法对陀螺IV和人topo I和II对陀螺酶抑制超螺旋和松弛的IC 50值的确定。 在两种测定中均获得了相似的抑制谱和IC 50值。